Description :
Microscopic examination of clinical samples for acid-fast bacilli using the Ziehl-Neelsen (ZN) stain has been a standard diagnostic tool and is used globally for rapid TB diagnosis. Microscopy can detect 60% to 70% of culture-positive samples with a lower limit of detection of 5 × 103 organisms/mL.
Ziehl-Neelsen stain, the current feasible microbiological method for the diagnosis of tuberculosis, often needs a large amount of cerebrospinal fluid specimen but shows a low detection rate of M. tuberculosis.
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